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Dr. Rath Health Foundation

Dr. Rath Health Foundation

 

Antitumorigenic Activity of a Natural Anti-Cancer Formula in Human Breast Cancer Lines MDA-MB-231 and MCF-7.

Roomi, M.W., Ivanov, V., Rath, M. and Niedzwiecki, A.

Presented at: The 8th Annual Multidisciplinary Symposium on Breast Disease, Amelia Island, FL, February 13-16, 2003'
Published in: Conference Proceedings

The 8th Annual Multidisciplinary Symposium on Breast Disease

Worldwide, breast cancer is the most prevalent cancer in women. Metastasis potential and invasiveness of breast cancer are attributed to up-regulation of matrix metalloproteinases (MMPs). In the current study, we studied the effect of a natural anti-cancer formula on invasive potential of human breast cancer cell lines.

The natural anti-cancer formula is a specific mixture of lysine, proline, ascorbic acid and epigallocatechin, which we have shown previously that have a very potent synergistic antitumor activity through inhibiting extracellular matix invasion by cancer cells.

In this study we tested a natural anti-cancer formula’s effect on estrogen-receptor positive (ER+) MCF-7 and estrogen-receptor negative (ER-) MDA-MB-231 breast cancer cell lines. Metastatic parameters: expression of MMPs by zymography, cellular invasion through Matrigel and proliferation/cytotoxicity by MTT assay were studied. Invasion of MBA-MD-231 through matrigel was inhibited by 50%, 60% and 95% by 10, 50 and 100 ug/ml of a natural anti-cancer formula respectively. A natural anti-cancer formula was not toxic to MDA-MB-231 at 10ug/ml, showed slight toxicity at 100ug/ml.

However, it exhibited significant toxicity at 1000 ug/ml. Neither MMP-2 nor MMP-9 were detected by gelatinase zymography. In contrast, a natural anti-cancer formula was not toxic to MCF-7 at 10, 50, 100 and 500 ug/ml, and exhibited slight toxicity at 1000 ug/ml. Interestingly, MCF-7 was not invasive through Matrigel and did not express any MMP activity. These results suggest that a natural anti-cancer formula is valuable and promising candidate for ER-, MDA-MB-231 breast cancer cells. Further studies will be required for ER+ , MCF-7 breast cancer cells to determine the efficacy of a natural anti-cancer formula.

Poster:

Poster